Volume 2 Issue 2 | 2025 | View PDF
Paper Id:IJMSM-V2I2P114
doi: 10.71141/30485037/V2I2P114

Molecular Characterization of Bacteria Isolated from Soil Sample Collected from Oyscatech Pig House

Bamigboye Oluwabunmi Florence, Amusat Afusat Ibiwumi, Bakare Bisola Adebola, Aribisala, Lukman Aderemi, A.O. Olalekan, David Timilehin Dorcas, Ntagbu Folasade Gift, Ogunwole Michael Ogunmola

Citation:
Bamigboye Ouwabunmi Florence, Amusat Afusat Ibiwumi, Bakare Bisola Adebola, Aribisala, Lukman Aderemi, A.O. Olalekan, David Timilehin Dorcas, Ntagbu Folasade Gift, Ogunwole Michael Ogunmola, "Molecular Characterization of Bacteria Isolated from Soil Sample Collected from Oyscatech Pig House" International Journal of Multidisciplinary on Science and Management, Vol. 2, No. 2, pp. 150-158, 2025.

Abstract:
This investigation describes the isolation and molecular characterization of bacteria isolated from soil samples collected from Pig House in Oyo State College of Agriculture and Technology, Igboora. Isolation from soil samples was carried out and the isolated organisms were characterized using biochemical tests and molecular characterization. Two isolates were Gram-negative while sixteen were Gram-positive they are: Staphylococcus aureus 38.8 %, Staphylococcus epidermidis 16.7%, Streptococcus spp 11.1 %, Neisseria spp 11.1 %, Enterococcus spp 5.6 %, Micrococcus luteus, 5.6% Salmonella enterica 5.6% and Escherichia coli 5.6%. DNA extraction was carried out using Zyms quick DNA Bacteria. The Polymerase Chain Reaction (PCR) was used Phylogenetically to predict the likely bacteria DNA of the selected organism and the relationship between them when compare with the GenBank and the isolates were identified as Escherichia coli and Salmonella enterica. This study provided information on some bacteria harbored by pig house soil which could be a medium of transmission of diseases to animals, handlers and the community. Therefore, proper sanitary measures should be adopted in the pig house to avoid transmission of diseases from the pig to the human through handling and when consume as meat.

Keywords: DNA, molecular characterization, Pig house, isolation, Phylogenetic, Polymerase Chain Reaction.

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